Culture Based Isolation of Pathogenic Bacteria Associated with Respiratory Disease Complex in Broiler with Special Reference to Ornithobacterium rhinotracheale from India

Abstract

In this study, isolation and identification of pathogenic bacteria, with special reference to Ornithobacterium rhinotracheale associated with respiratory diseases complex (RDC), were performed from a total of 60 biomaterials collected from healthy and diseased broilers of commercially reared farms in and around Palanpur, Banaskantha, Gujarat. Prevalence of RDC was 6.67% and 50.00% in apparently healthy and diseased broilers respectively. The incidence of E.coli, Staphylococcus spp., Ornithobacterium rhinotracheale, Pasteurella spp. and Klebsiella spp. were 8 (47.06%), 4 (23.53%), 3 (17.65%), 1 (5.88%) and 1 (5.88%) in broilers respectively. Highest bacteria were isolated from lung (58.33) followed by trachea (41.66). Ornithobacterium rhinotracheale, one of the causative agents of the emerging respiratory diseases complex of broiler could be isolated either singly or concurrently with other bacteria such as Escherichia coli, Klebsiella spp. and Pasteurella spp. indicating its possible etiological role in respiratory disease.

Keywords

• respiratory diseases complex
• isolation
• ornithobacterium rhinotracheale
• broilers