FucP promotes the pathogenicity of Edwardsiella piscicida to infect zebrafish

Xinya Zhang; Maocang Yan; Cuimin Mu; Jiayan Wu; Jiakang Chen; Guangchen Pan; Xuepeng Wang

Aquaculture Reports (Jul 2021)

FucP promotes the pathogenicity of Edwardsiella piscicida to infect zebrafish

Xinya Zhang,
Maocang Yan,
Cuimin Mu,
Jiayan Wu,
Jiakang Chen,
Guangchen Pan,
Xuepeng Wang

Affiliations

Xinya Zhang: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention & Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian, 271018, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 272000, PR China
Maocang Yan: Zhejiang Mariculture Research Institute, Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource (Wenzhou), Wenzhou Key Laboratory of Marine Genetics and Breeding, Wenzhou, 325005, PR China
Cuimin Mu: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention & Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian, 271018, PR China
Jiayan Wu: Zhejiang Mariculture Research Institute, Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource (Wenzhou), Wenzhou Key Laboratory of Marine Genetics and Breeding, Wenzhou, 325005, PR China
Jiakang Chen: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention & Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian, 271018, PR China
Guangchen Pan: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention & Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian, 271018, PR China
Xuepeng Wang: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention & Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian, 271018, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 272000, PR China; Corresponding author at: Shandong Agricultural University, Taian, 271018, PR China.

Journal volume & issue: Vol. 20
p. 100665

Abstract

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Edwardsiella piscicida (E. piscicida) is an important zoonotic pathogen that mostly infects fish by colonizing the intestine. Fucose within the intestine could regulate the pathogenicity of some intestinal pathogens. Here we identified the function of fucose permease in E. piscicida and its physiological influence. The results showed that E. piscicida grew faster in the presence of l-fucose. The fucose permease gene fucP mutant strain ΔfucP significantly decreased penetration and motility in mucin compared with the wild-type strain. The gene expression levels of esaB, esaD, esaL and esaK in ΔfucP had significantly downregulated when compared with the wild-type strain. Most importantly, the survival rate of zebrafish after being infected with ΔfucP was significantly higher than those of the wild type. In a word, fucose promoted the growth and motility of E. piscicida and the fucP gene mutant decreased the pathogenicity of E. piscicida in zebrafish.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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