PLoS ONE (Jan 2015)

Mechanisms involved in the anti-inflammatory action of a polysulfated fraction from Gracilaria cornea in rats.

  • Chistiane Oliveira Coura,
  • Ricardo Basto Souza,
  • José Ariévilo Gurgel Rodrigues,
  • Edfranck de Sousa Oliveira Vanderlei,
  • Ianna Wivianne Fernandes de Araújo,
  • Natássia Albuquerque Ribeiro,
  • Annyta Fernandes Frota,
  • Kátia Alves Ribeiro,
  • Hellíada Vasconcelos Chaves,
  • Karuza Maria Alves Pereira,
  • Rodrigo Maranguape Silva da Cunha,
  • Mirna Marques Bezerra,
  • Norma Maria Barros Benevides

DOI
https://doi.org/10.1371/journal.pone.0119319
Journal volume & issue
Vol. 10, no. 3
p. e0119319

Abstract

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The anti-inflammatory mechanisms of the sulfated polysaccharidic fraction obtained from red marine alga Gracilaria cornea (Gc-FI) were investigated using a paw edema model induced in rats by different inflammatory agents (carrageenan, dextran, serotonin, bradykinin, compound 48/80 or L-arginine). Gc-FI at the doses of 3, 9 or 27 mg/kg, subcutaneously--s.c., significantly inhibited rat paw edema induced by carrageenan and dextran, as confirmed by myeloperoxidase and Evans' blue assessments, respectively. Gc-FI (9 mg/kg, s.c.) inhibited rat paw edema induced by histamine, compound 48/80 and L-arginine. Additionally, Gc-FI (9 mg/kg, s.c.) inhibited Cg-induced edema in animals with intact mast cells but did not inhibit that with degranulated mast cells by compound 48/80, revealing a protective role on mast cell membranes. Gc-FI down-regulated the IL-1β, TNF-α and COX-2 mRNA and protein levels compared with those of the carrageenan group, based on qRT-PCR and immunohistochemistry analyses. After inhibition with ZnPP IX, a specific heme oxygenase-1 (HO-1) inhibitor, the anti-inflammatory effect of Gc-FI was not observed in Cg-induced paw edema, suggesting that the anti-inflammatory effect of Gc-FI is, in part, dependent on the integrity of the HO-1 pathway. Gc-FI can target a combination of multiple points involved in inflammatory phenomena.