The Influence of Some Phytobiotics on Haematological and Some Biochemical Indices at Oreochromis Niloticus–Linnaeus, 1758

Abstract

The aim of this research was to evaluated the influence of some phytobiotics on haematological profile, leukocyte reaction and some biochemical indices at Oreochromis niloticus species, reared in a recirculating aquaculture system. This experiment was conducted six weeks. The experimental variants were: V1–control; V2–1% Rosmarinus officinali/kg feed; V3–1% Hippophae rhamnoides/kg feed and V4–1% Zingiber officinale/kg feed. Blood was analyzed using standard techniques. At the end of the experiment the following parameters were determined: RBCc (x106cells/µL), Hb (g/dL), PVC (%), MCV (µm3), MCH (pg), MCHC (g/dL), TP (g/dL), GLU (mg/dL), cortisol (ng/mL), lysozyme activity (U/mL), absolute number of blood cells (x103 cells/µL) and leukogram (%). The results showed that the administration in feed of some phytobiotics lead to signifiant differences (p<0.05) of following parameters: RBCc (x106cells/µL), MCV (µm3), glucose (mg/dL), lysozyme activity (U/mL), monocyte (%) and in absolute number of leukocytes, lymphocytes and monocytes. In conclusion, due to decreasing of RBCc, PVC, Hb, MCHC, cortisol, GLU and due to normal concentration of TP, we can say that the administration of sea buckthorn and ginger, but even rosemary administration, in diet improves the physiological status at Oreochromis niloticus species.

Keywords

• haematological profile
• leukogram
• lysozyme activity
• oreochromis niloticus
• phytobiotics
• recirculating aquaculture system