Frontiers in Microbiology (Aug 2022)
New rapid detection by using a constant temperature method for avian leukosis viruses
- Xiuhong Wu,
- Xiuhong Wu,
- Xiuhong Wu,
- Fengsheng Chu,
- Fengsheng Chu,
- Fengsheng Chu,
- Luxuan Zhang,
- Sheng Chen,
- Sheng Chen,
- Sheng Chen,
- Liguo Gao,
- Liguo Gao,
- Liguo Gao,
- Hao Zhang,
- Hao Zhang,
- Haohua Huang,
- Haohua Huang,
- Jin Wang,
- Jin Wang,
- Mengjun Chen,
- Mengjun Chen,
- Zi Xie,
- Zi Xie,
- Zi Xie,
- Feng Chen,
- Feng Chen,
- Feng Chen,
- Xinheng Zhang,
- Xinheng Zhang,
- Xinheng Zhang,
- Qingmei Xie,
- Qingmei Xie,
- Qingmei Xie
Affiliations
- Xiuhong Wu
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xiuhong Wu
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xiuhong Wu
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Fengsheng Chu
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Fengsheng Chu
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Fengsheng Chu
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Luxuan Zhang
- School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China
- Sheng Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Sheng Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Sheng Chen
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Liguo Gao
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Liguo Gao
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Liguo Gao
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Hao Zhang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hao Zhang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Haohua Huang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Haohua Huang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Jin Wang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Jin Wang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Mengjun Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Mengjun Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Zi Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zi Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Zi Xie
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Feng Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Feng Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Feng Chen
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Xinheng Zhang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xinheng Zhang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xinheng Zhang
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Qingmei Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingmei Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Qingmei Xie
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2022.968559
- Journal volume & issue
-
Vol. 13
Abstract
The avian leukemia virus causes avian leukemia (AL), a severe immunosuppressive disease in chickens (ALV). Since the 1990s, the diversity of ALV subpopulations caused by ALV genome variation and recombination, and the complexity of the infection and transmission, with currently no effective commercial vaccine and therapeutic for ALV, has resulted in severe economic losses to the chicken business in various parts of the world. Therefore, as a key means of prevention and control, an effective, rapid, and accurate detection method is imperative. A new real-time reverse transcription recombinase-aided amplification (RT-RAA) assay for ALV with rapid, highly specific, low-cost, and simple operational characteristics have been developed in this study. Based on the amplification of 114 base pairs from the ALV P12 gene, real-time RT-RAA primers and a probe were designed for this study. The lowest detection line was 10 copies of ALV RNA molecules per response, which could be carried out at 39°C in as fastest as 5 min and completed in 30 min, with no cross-reactivity with Marek's disease virus, avian reticuloendothelial virus, Newcastle disease virus, infectious bronchitis virus, infectious bursal disease virus, infectious laryngotracheitis virus, and avian influenza virus. Furthermore, the kappa value of 0.91 (>0.81) was compared with reverse transcription–polymerase chain reaction (RT-PCR) for 44 clinical samples, and the coefficients of variation were within 5.18% of the repeated assays with three low-level concentration gradients. These results indicate that using a real-time RT-RAA assay to detect ALV could be a valuable method.
Keywords
- avian leukosis viruses
- p12
- real-time reverse-transcription recombinase-aided amplification assay
- detection by constant temperature
- clinical diagnosis
