Reproducible Method for 1-Methylcylopropene (1−MCP) Application and Quantitation for Post-Harvest Research

Abstract

Actively combating post-harvest food spoilage and waste can dramatically increase the efficiency of food utilization worldwide. In climacteric fruits, chemical treatments such as 1−MCP are an effective way of reducing post-harvest spoilage and waste by inhibiting the fruit’s ability to perceive ethylene. 1−MCP treatment is increasingly being used to explore the complex nature of ripening physiology at a fundamental level; however, differences in application and quantitation methods create difficulties in comparing conclusions across studies. Here, we report an effective and reproducible method for 1−MCP application and quantitation for small–medium-sized research applications. By use of surrogate alkene standards 1-butene and cis-2-butene, the highly volatile and elusive 1−MCP molecule can be identified and quantified by gas chromatography and subsequent standard curves may be developed. It is hoped that the methodology outlined here can help standardize consistent 1−MCP application for post-harvest research without excessive investment in specialized equipment.

Keywords

• fruit ripening
• post-harvest
• ethylene
• 1-methylcylcopropene