Nature Communications (Jul 2022)
A unique class of Zn2+-binding serine-based PBPs underlies cephalosporin resistance and sporogenesis in Clostridioides difficile
- Michael D. Sacco,
- Shaohui Wang,
- Swamy R. Adapa,
- Xiujun Zhang,
- Eric M. Lewandowski,
- Maura V. Gongora,
- Dimitra Keramisanou,
- Zachary D. Atlas,
- Julia A. Townsend,
- Jean R. Gatdula,
- Ryan T. Morgan,
- Lauren R. Hammond,
- Michael T. Marty,
- Jun Wang,
- Prahathees J. Eswara,
- Ioannis Gelis,
- Rays H. Y. Jiang,
- Xingmin Sun,
- Yu Chen
Affiliations
- Michael D. Sacco
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Shaohui Wang
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Swamy R. Adapa
- Department of Global and Planetary Health, USF Genomics Program, Global Health and Infectious Disease Center, College of Public Health, University of South Florida
- Xiujun Zhang
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Eric M. Lewandowski
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Maura V. Gongora
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Dimitra Keramisanou
- Department of Chemistry, University of South Florida
- Zachary D. Atlas
- School of Geosciences, University of South Florida
- Julia A. Townsend
- Department of Chemistry and Biochemistry, The University of Arizona
- Jean R. Gatdula
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Ryan T. Morgan
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Lauren R. Hammond
- Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida
- Michael T. Marty
- Department of Chemistry and Biochemistry, The University of Arizona
- Jun Wang
- Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey
- Prahathees J. Eswara
- Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida
- Ioannis Gelis
- Department of Chemistry, University of South Florida
- Rays H. Y. Jiang
- Department of Global and Planetary Health, USF Genomics Program, Global Health and Infectious Disease Center, College of Public Health, University of South Florida
- Xingmin Sun
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- Yu Chen
- Department of Molecular Medicine, Morsani College of Medicine, University of South Florida
- DOI
- https://doi.org/10.1038/s41467-022-32086-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
Antibiotics of the β-lactam class inhibit bacterial cell wall synthesis by targeting penicillin-binding proteins (PBPs). Here, Sacco et al. study the four PBPs present in the pathogen C. difficile, revealing unique structural features and shedding light on the mechanisms underlying β-lactam resistance in this organism.
