Molecular identification of oxalate-degrading lactobacillus in patients with calcium oxalate urolithiasis

Abstract

Background: Excessive use of diets with high level of oxalate causes an increase in urinary oxalate and leads to the formation of oxalate calcium stone. The aim of this study was evaluation and isolation of an oxalate-degrading bacterium in patients with kidney calcium oxalate stone. Materials and Methods: This cross sectional-descriptive study was carried out on stool and urine samples of 100 healthy individuals and 100 patients with calcium oxalate stone disease in Motahari hospital of Jahrom. The Oxalate-degrading bacteria enriched and cultured in specific medium and were identified by using biochemical tests and 16S rRNA molecular method. Moreover, the presence of oxc and frc genes in isolated bacteria was detected. Results: Lactobacilli were isolated from 80% of healthy individuals and 48% of patients with calcium oxalate stone disease. In 60(46.88%) cases of lactobacilli, oxc gene and 14 (10.94%) cases, frc genes were identified. There was a significant difference between level of urinary oxalate in patients with kidney stone and healthy individuals. But, oxalate – degrading lactobacilli colonization were significantly higher in healthy individuals in comparison with patients with kidney stone. Conclusion: Since the reduction of oxalate- degrading bacteria is one of the reasons of hyperoxaluria and urolithiasis, using these bacteria is recommended for reducing kidney stones.

Keywords

• oxalate-degrading bacteria
• lactobacilli
• calcium oxalate
• kidney stone