Development and Evaluation of Atorvastatin Calcium Nanovesicular Niosomal Gel for the Treatment of Periodontitis

Abstract

Nowadays, smoking and tobacco-related habits (chewing tobacco) are recognized as the most frequent environmental risk factor for periodontal disorders. Atorvastatin calcium (ATV) is a well-known lipid-lowering drug, but recent studies have discussed its pleiotropic effects, including anti-inflammatory properties, anti-bacterial properties, etc. This anti-inflammatory effect can be studied as an adjunct for scaling and root planing (a non-surgical process to remove dental tartar and smooth root surfaces) for periodontic problems. The goal of the study is to formulate and evaluate ATV-niosomes, introduce them in a gel-based formulation by utilizing an appropriate gelling agent, and evaluate them for various parameters. The niosomal vesicles were prepared using the thin-film hydration method. Gel was prepared using the dispersion method, and an in vitro drug release study was conducted using a Franz diffusion cell. According to the results evaluated, ATV niosomal gels loaded with different concentrations of Carbopol 934 were effectively produced utilizing ATV-niosomes that were formulated through the thin-film hydration process using cholesterol and Span 60. The ATV-niosomes showed the highest entrapment efficiency, up to 84%, and the zeta potential (−18 mV) and PDI (0.106) showed stable and homogenous behavior in the vesicles formed. The performance of the optimized gel containing 1% Carbopol 934 showed in vitro release of up to 8 h following zero-order release. The gel also proved to have antimicrobial activity against S. aureus and P. aeruginosa. Therefore, we conclude that 1% Carbopol 934 gel comprising ATV-niosomes showed a prolonged effect compared to plain ATV and can effectively work to improve the periodontal condition as an adjunct to scaling and root planing.

Keywords

• niosomes
• atorvastatin calcium
• periodontitis
• gel
• Carbopol 934