Mitochondrial DNA. Part B. Resources (Jan 2020)

Complete chloroplast genome sequence of Pueraria lobata (Willd.) Ohwi (Fabaceae): a traditional Chinese medicinal herb

  • Jiahui Sun,
  • Ling Wang,
  • Yuan Yuan,
  • Rongrong Zhou,
  • Haiyan Duan,
  • Yuping Zhao

DOI
https://doi.org/10.1080/23802359.2019.1694850
Journal volume & issue
Vol. 5, no. 1
pp. 25 – 26

Abstract

Read online

Pueraria lobata is a traditional Chinese herb which has been used medically since ancient times. In this study, we sequenced the complete chloroplast genome of P. lobata based on next-generation sequencing and used the data to assess genomic resources. The chloroplast genome of P. lobata is 153,411 bp in length consisting of large and small single-copy regions of length 84,142 and 17,989 bp, separated by two IR regions of 25,640 bp. The overall GC content was 35.4%. De novo assembly and annotation showed the presence of 112 unique genes with 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. A maximum-likelihood phylogenomic analysis showed that Pueraria was sister to Pediomelum + Glycine.

Keywords