Live imaging and conditional disruption of native PCP activity using endogenously tagged zebrafish sfGFP-Vangl2

Maria Jussila; Curtis W. Boswell; Nigel W. Griffiths; Patrick G. Pumputis; Brian Ciruna

doi:10.1038/s41467-022-33322-9

Nature Communications (Sep 2022)

Live imaging and conditional disruption of native PCP activity using endogenously tagged zebrafish sfGFP-Vangl2

Maria Jussila,
Curtis W. Boswell,
Nigel W. Griffiths,
Patrick G. Pumputis,
Brian Ciruna

Affiliations

Maria Jussila: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children
Curtis W. Boswell: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children
Nigel W. Griffiths: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children
Patrick G. Pumputis: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children
Brian Ciruna: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children

DOI: https://doi.org/10.1038/s41467-022-33322-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Planar cell polarity (PCP) is critical for tissue-wide coordination and successful development. Here Jussila et al. generate a GFP-Vangl2 fusion for live imaging and discover a surprising directionality to the intercellular propagation of cell polarity, and ultimately link PCP defects with idiopathic scoliosis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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