<i>In vitro</i> Study of Biological Properties of SARS-CoV-2 Coronavirus Strains Related to Various Genetic Variants

Abstract

The aim of the research was to study reproduction features of SARS-CoV-2 coronavirus strains of various genetic lines on Vero and Vero E6 cell culture.Materials and methods. The SARS-CoV-2 virus strains related to the variants of concern (VOC) circulating in the territory of the Russian Federation were used in the research. The strains of the SARS-CoV-2 virus were deposited in the State Collection of Pathogens of Viral Infections and Rickettsioses at the FBIS SSC VB “Vector” of the Rospotrebnadzor. The experiments were carried out on Vero and Vero E6 cell cultures. The dynamics of infectious virus accumulation was determined by titration of culture fluid samples 24, 48, 72, 96 hours after infection (MOI – from 1 to 0,00001 CPE50/cell). Plaque formation was studied on Vero E6 cell culture under 0.2 % agar coating. Image analysis and plaque size calculation were performed using GIMP (GNU Image Manipulation Program).Results and discussion. The study describes the dynamics of accumulation of infectious virus in the culture fluid depending upon multiplicity of infection for the strains of SARS-CoV-2 virus belonging to different genetic lines. Differences in the morphology of plaques on the monolayer of Vero E6 cell culture under agar coating are shown. SARS-CoV-2 virus strains related to Alfa and Delta VOC demonstrate maximum reproduction rate among the studied strains (infectious titer is higher than 7 lg TCID50/100µl). Omicron VOC forms small plaques under agar coating and at a low multiplicity of infection has a low reproduction rate. Thus, SARS-CoV-2 virus strains belonging to different genetic lines have significant differences in the rate of reproduction on Vero and Vero E6 cell culture.

Keywords

• sars-cov-2 coronavirus
• voc
• vero cell culture
• vero e6 cell culture
• virus titer
• plaque formation