Frontiers in Immunology (Sep 2022)

The pyroptosis-related gene signature predicts prognosis and indicates the immune microenvironment status of chronic lymphocytic leukemia

  • Yeqin Sha,
  • Yeqin Sha,
  • Yeqin Sha,
  • Rui Jiang,
  • Rui Jiang,
  • Rui Jiang,
  • Yi Miao,
  • Yi Miao,
  • Yi Miao,
  • Shuchao Qin,
  • Shuchao Qin,
  • Shuchao Qin,
  • Wei Wu,
  • Wei Wu,
  • Wei Wu,
  • Yi Xia,
  • Yi Xia,
  • Yi Xia,
  • Li Wang,
  • Li Wang,
  • Li Wang,
  • Lei Fan,
  • Lei Fan,
  • Lei Fan,
  • Hui Jin,
  • Hui Jin,
  • Hui Jin,
  • Wei Xu,
  • Wei Xu,
  • Wei Xu,
  • Jianyong Li,
  • Jianyong Li,
  • Jianyong Li,
  • Jianyong Li,
  • Huayuan Zhu,
  • Huayuan Zhu,
  • Huayuan Zhu

DOI
https://doi.org/10.3389/fimmu.2022.939978
Journal volume & issue
Vol. 13

Abstract

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Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world with great heterogeneity. Pyroptosis has recently been recognized as an inflammatory form of programmed cell death (PCD) and shares a close relationship with apoptosis. Although the role of apoptosis in CLL was comprehensively studied and successfully applied in clinical treatment, the relationship between pyroptosis genes and CLL remained largely unknown. In this study, eight differentially expressed pyroptosis-related genes (PRGs) were identified between CLL and normal B cells. In order to screen out the prognostic value of differentially expressed PRGs, univariate and multivariate Cox regression analyses were conducted and a risk model with three PRG signatures (GSDME, NLRP3, and PLCG1) was constructed. All CLL samples were stratified into high- and low-risk subgroups according to risk scores. The risk model showed high efficacy in predicting both overall survival (OS) and time to first treatment (TTFT). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) showed the dysregulation of immune and inflammatory response in the high-risk group. Single-sample GSEA (ssGSEA) of immune cell infiltration and the activity of immune-related pathways also displayed decreased antitumor immunity in the high-risk group. In conclusion, PRGs are of prognostic value in CLL and may play important roles in tumor immunity, and the underlying relationship between PRGs and CLL needs to be explored further.

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