PLoS ONE (Jan 2019)

HBV preS deletion mapping using deep sequencing demonstrates a unique association with viral markers.

  • Yuichiro Suzuki,
  • Shinya Maekawa,
  • Nobutoshi Komatsu,
  • Mitsuaki Sato,
  • Akihisa Tatsumi,
  • Mika Miura,
  • Shuya Matsuda,
  • Masaru Muraoka,
  • Natsuko Nakakuki,
  • Fumitake Amemiya,
  • Shinichi Takano,
  • Mitsuharu Fukasawa,
  • Yasuhiro Nakayama,
  • Tatsuya Yamaguchi,
  • Taisuke Inoue,
  • Tadashi Sato,
  • Minoru Sakamoto,
  • Atsuya Yamashita,
  • Kohji Moriishi,
  • Nobuyuki Enomoto

DOI
https://doi.org/10.1371/journal.pone.0212559
Journal volume & issue
Vol. 14, no. 2
p. e0212559

Abstract

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AimDeletions are observed frequently in the preS1/S2 region of hepatitis B virus (HBV) genome, in association with liver disease advancement. However, the most significant preS1/S2 region and its influences on viral markers are unclear.MethodsThe preS1/S2 HBV regions of 90 patients without antiviral therapy were subjected to deep sequencing and deleted regions influencing viral markers were investigated.ResultsFrom the deletion frequency analysis in each patient, deletions were observed most frequently in the preS2 codon 132-141 region. When the patients were divided into three groups (0-0.1%: n = 27, 0.1%-10%: n = 34, 10-100%: n = 29), based on the deletion frequency, FIB-4 (p ConclusionThe preS codon.132-141 deletions have a significant influence on the clinical characteristics and viral markers, even when present as a minor population. Importantly, the preS codon 132-141 deletions have a clear influence on the viral life cycle and pathogenesis.