Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

Melanie R. Silvis; Manohary Rajendram; Handuo Shi; Hendrik Osadnik; Andrew N. Gray; Spencer Cesar; Jason M. Peters; Cameron C. Hearne; Parth Kumar; Horia Todor; Kerwyn Casey Huang; Carol A. Gross

doi:10.1128/mBio.02561-21

mBio (Oct 2021)

Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

Melanie R. Silvis,
Manohary Rajendram,
Handuo Shi,
Hendrik Osadnik,
Andrew N. Gray,
Spencer Cesar,
Jason M. Peters,
Cameron C. Hearne,
Parth Kumar,
Horia Todor,
Kerwyn Casey Huang,
Carol A. Gross

Affiliations

Melanie R. Silvis: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Manohary Rajendram: Department of Bioengineering, Stanford University, Stanford, California, USA
Handuo Shi: ORCiD; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
Hendrik Osadnik: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Andrew N. Gray: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Spencer Cesar: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
Jason M. Peters: ORCiD; Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin—Madison, Madison, Wisconsin, USA
Cameron C. Hearne: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Parth Kumar: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Horia Todor: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Kerwyn Casey Huang: ORCiD; Department of Bioengineering, Stanford University, Stanford, California, USA
Carol A. Gross: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA

DOI: https://doi.org/10.1128/mBio.02561-21
Journal volume & issue: Vol. 12, no. 5

Abstract

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Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.

Published in mBio

ISSN: 2161-2129 (Print); 2150-7511 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/mbio

About the journal