Emerging Infectious Diseases (Feb 2020)

Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness

  • Heather P. McLaughlin,
  • Julia V. Bugrysheva,
  • Andrew B. Conley,
  • Christopher A. Gulvik,
  • Blake Cherney,
  • Cari B. Kolton,
  • Chung K. Marston,
  • Elke Saile,
  • Erin Swaney,
  • David Lonsway,
  • Amy S. Gargis,
  • Thiphasone Kongphet-Tran,
  • Christine Lascols,
  • Pierre Michel,
  • Julie Villanueva,
  • Alex R. Hoffmaster,
  • Jay E. Gee,
  • David Sue

DOI
https://doi.org/10.3201/eid2602.191351
Journal volume & issue
Vol. 26, no. 2
pp. 358 – 361

Abstract

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Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.

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