Age related histopathological and immunohistochemical changes in horse brains

Abstract

The aim of this study was to investigate age dependent pathological changes in horse brains. In the study, 10 mature horses aged between 10-16 years, 13 old horses aged between 17-21 years old and 7 young horses between 4-7 years old were examined macroscopically, histopathologically and immunohistohemically. As compared young horse brains; ventricular dilatation, satellitosis, neuronal vacuolization, status spongiosis, ventricular dilatation and ependymal undulation, calcium deposits and axonal swellings were seen in old horse brains. Immunohistochemical staining was obtained with Glial Fibrillary Acidic Protein (GFAP), 2",3"-Cyclic Nucleotide 3"-Phosphodiesterase (CNPase), neuron-specific enolase (NSE), β-amyloid protein (Aβ) antibodies to indicate age related changes in brains.

Keywords

• brain
• histopathology
• immunohistochemistry
• old horse