Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent

Abstract

Background: The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls. Aim: This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls. Methods: Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1 mg/100 mL, 0.15 mg/100 mL, and 0.2 mg/100 mL, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (p [Open Vet J 2024; 14(8.000): 2040-2048]

Keywords

• bali bull
• egcg
• kinematics
• livestock and gene bank
• sperm quality