Journal of Islamic International Medical College (Mar 2023)
Protective Effects of Turmeric (Curcuma Longa) Against Methotrexate Induced Liver Toxicity in The Albino Mice
Abstract
Objective: To study the protective effects of Turmeric against hepatic damage brought on by Methotrexate. Study Design: Randomized controlled trial. Place and Duration of Study: This experimental research was conducted at Animal House of Institute of Basic Medical Science (IBMS), Khyber Medical University, Peshawar from 10June 2017 to 25 June 2018. Materials and Methods: A total of 28 adult albino mice were divided into four groups. Group A was control group. Control group received no medication. Group B received a daily dose of Turmeric extract (400 mg/kg) for 14 days. Group C received an intraperitoneally (I.P) injection of Methotrexate (40 mg/kg) on day 7. Group D received oral administration of Turmeric extract (400mg/kg) for 14 days and injection Methotrexate (40mg/kg) was administered intraperitoneally (I.P) on day 7. All mice were sacrificed on day 14. Weight of liver of all the animal was recorded. For data analysis Statistical package for social sciences (SPSS) version 21 was used. Quantitative variables were expressed as mean ±standard deviation and the significant difference was assessed using ANOVA test. The Chi square test was used to determine statistical significance based on the categorical variables. P value <.05 was significant statistically. Results: On microscopic examination of liver tissue, abundant inflammatory cells were observed around the portal area in all mice (100%) of Methotrexate group (Group C) with increase in liver weight. In Methotrexate +Turmeric group (Group D) few inflammatory cells were observed with slight decrease in liver weight as compared to Group C in all mice (100%). Conclusion: The results of this study revealed that Turmeric (Curcuma Longa) treatment protects liver tissue against Methotrexate-induced damage.
