JTO Clinical and Research Reports (Jul 2023)

B-Cell Infiltrate in the Tumor Microenvironment Is Associated With Improved Survival in Resected Lung Adenocarcinoma

  • Ibiayi Dagogo-Jack, MD,
  • Ivan Valiev, MD,
  • Nikita Kotlov, MS,
  • Anna Belozerova, MD,
  • Aleksandra Lopareva, MS, MD,
  • Anna Butusova, MS,
  • Naira Samarina, PhD,
  • Alexandra Boyko, MS,
  • Zhongmin Xiang, PhD,
  • Monique Johnson, BS,
  • Sandrine Degryse, PhD,
  • Florence K. Keane, MD,
  • Lecia V. Sequist, MD,
  • Michael Lanuti, MD,
  • Nathan Fowler, MD,
  • Mari Mino-Kenudson, MD,
  • Alexander Bagaev, PhD

Journal volume & issue
Vol. 4, no. 7
p. 100527

Abstract

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Introduction: Relapse is common after resection of lung adenocarcinoma (LUAD). Features of the tumor microenvironment (TME) which influence postsurgical survival outcomes are poorly characterized. Here, we analyzed the TME of more than 1500 LUAD specimens to identify the relationship between B-cell infiltration and prognosis. Methods: Whole exome sequencing and bulk RNA sequencing were performed on LUADs and adjacent normal lung tissue. Relapse-free survival and overall survival (OS) were retrospectively correlated with characteristics of the tumor and TME in three data sets. Results: High B-cell content (defined as >10% B cells) was associated with improved OS in both a The Cancer Genome Atlas–resected LUAD data set (p = 0.01) and a separate institutional stage II LUAD data set (p = 0.04, median not reached versus 89.5 mo). A validation cohort consisting of pooled microarray data representing more than 1400 resected stage I to III LUADs confirmed the association between greater B-cell abundance, specifically higher B-cell expression, and longer postsurgical survival (median OS 90 versus 71 mo, p < 0.01). Relapse-free survival was longer for patients with adenocarcinomas with high B-cell content across data sets, but it did not reach statistical significance. Subcategorization of B-cell subsets indicated that high naive B-cell content was most predictive of survival. There was no correlation between programmed death-ligand 1 expression, lymphoid aggregates, or overall immune infiltrate density and survival outcomes across the cohorts. Conclusions: The growing adjuvant immunotherapy repertoire has increased the urgency for identifying prognostic and predictive biomarkers. Comprehensive profiling of more than 1500 LUADs suggests that high tumor-infiltrating B-cell content is a favorable prognostic marker.

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