Morphological and genetic demonstration of Cephalopina titillator in dromedary camels

Abstract

Background: Cephalopina titillator is one of the most important parasites, which infests the upper respiratory tract of camels leading to deteriorating health effects, substantial economic losses and even death. Aim: This study aimed was to detect the prevalence rate of C. titillator in slaughtered camels, determining its morphology using the electron microscope, and confirming its species by the molecular phylogeny. Methods: A total of 200 slaughtered camels at different areas in Al Muthanna province (Iraq) were inspected visually to collect the parasite samples that identified initially based on their morphological characteristics. To confirm the parasite species, molecular phylogeny was conducted targeting the COX1 gene. Results: An overall 19.5% of study camels were found infested with C. tittlator. Based on light and electron microscopes, the larval stage of C. tittlator was shown numerous posterior spiracular pores, cephalo-pharyngeal skeleton, abdominal segments, spinulation in anterior ventral portion, no spines on final segment of abdomen, and rounded dorsal surface. Dorsoventrally, a slender and flattened shape with presence of 12 segments as well as widely separated antennal lobes and obligate mouth hooks were seen. Molecularly, all the tested samples were found positive by PCR. Additionally, some positive PCR products were sequenced, and reported in the NCBI-GenBank under the access numbers of OP218846, OP218847, and OP218845, OP218839, OP218840, OP218841, OP218842, OP218843, and OP218844. Sequence analysis revealed the obvious identity between the local isolates and the global NCBI-GenBank Iran isolate (MW136151.1). Conclusion: This study described precisely the morphology of C. titillator using the light and electron microscopes suggesting its role in appropriate identification and classification. Molecular examination demonstrated the importance of COX1 gene in identification of C. titillator and sequencing of the local isolates; however, additional molecular phylogenetic studies are needed to establish the evolutionary relationships among the oestrid group of insects with specialized habits and habitats. [Open Vet J 2024; 14(11.000): 2995-3003]

Keywords

• nsopharyngeal myiasis
• cephalopina titillator
• electron microscopy
• camels