Nature Communications (Jun 2021)

Assessment of protein–protein interfaces in cryo-EM derived assemblies

  • Sony Malhotra,
  • Agnel Praveen Joseph,
  • Jeyan Thiyagalingam,
  • Maya Topf

DOI
https://doi.org/10.1038/s41467-021-23692-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

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Here, the authors present Protein Interface-score (PI-score), a machine learning-based metric that has been trained on protein–protein interfaces’ features from high-resolution crystal structures. They use the PI-score to evaluate the protein–protein interfaces in more than 1000 PDB-deposited cryo-EM structures and show that it can be used as a complementary assessment tool for cryo-EM model validation.