DNA methylation directs microRNA biogenesis in mammalian cells

Ohad Glaich; Shivang Parikh; Rachel E. Bell; Keren Mekahel; Maya Donyo; Yodfat Leader; Ronna Shayevitch; Danna Sheinboim; Sivan Yannai; Dror Hollander; Ze’ev Melamed; Galit Lev-Maor; Gil Ast; Carmit Levy

doi:10.1038/s41467-019-13527-1

Nature Communications (Dec 2019)

DNA methylation directs microRNA biogenesis in mammalian cells

Ohad Glaich,
Shivang Parikh,
Rachel E. Bell,
Keren Mekahel,
Maya Donyo,
Yodfat Leader,
Ronna Shayevitch,
Danna Sheinboim,
Sivan Yannai,
Dror Hollander,
Ze’ev Melamed,
Galit Lev-Maor,
Gil Ast,
Carmit Levy

Affiliations

Ohad Glaich: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Shivang Parikh: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Rachel E. Bell: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Keren Mekahel: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Maya Donyo: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Yodfat Leader: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Ronna Shayevitch: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Danna Sheinboim: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Sivan Yannai: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Dror Hollander: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Ze’ev Melamed: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Galit Lev-Maor: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Gil Ast: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine
Carmit Levy: Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine

DOI: https://doi.org/10.1038/s41467-019-13527-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Long primary transcripts of microRNAs are co-transcriptionally cleaved by the enzyme Drosha. Here the authors suggest that DNA methylation in miRNA loci in mammalian cells increases Drosha binding, slowing RNA polymerase II elongation to enhance miRNA biogenesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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