Journal of Clinical and Diagnostic Research (Jul 2019)

The Potential Curative and Prophylactic Effects of Grape Seed Extract on Aspirin Induced Gastric Ulcer in Adult Male Albino Rats

  • Basma S AbdElatif,
  • Fatma El-Nabawia A El-Safty,
  • Ahmed S Zolfakar,
  • Asmaa S Essawy

DOI
https://doi.org/10.7860/JCDR/2019/41620.13016
Journal volume & issue
Vol. 13, no. 7
pp. AF01 – AF06

Abstract

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Introduction: Aspirin {Acetyl Salicylic Acid (ASA)} is a potent Nonsteroidal Anti-Inflammatory Drug (NSAID). Gastric ulcer associated with the use of aspirin is a major problem. Grape Seed Extract (GSE), has been shown to produce effective antioxidant effects. Aim: To evaluate the effect of grape seed extract on gastric ulcer induced by aspirin. Materials and Methods: Fifty adult rats were classified into: Group I (control group); Group II (GSE group), which received a single dose of GSE (250 mg/kg/day) by gavage for 10 days; Group III (Gastric ulcer group), which received (300 mg/kg) of aspirin by gavage, Group IV (Prophylactic group), which received GSE (250 mg/kg/day) by gavage for 10 days, one hour after administration of the last dose each rat received a single dose of aspirin (300 mg/kg) by gavage and Group V (Curative group), which received a single oral dose (300 mg/kg) of aspirin, five hours after administration of aspirin each rat received an oral dose (250 mg/kg) of GSE for 10 days. At the end of the study, the rats were anaesthetized and the stomach from all groups were extracted and examined. ANOVA test followed by a post-hoc test (LSD) were employed to compare the results of studied animal groups. Results: The gastric tissue of group III revealed extensive mucosal erosions and sloughing of gastric glands. GSE administration in the prophylactic group resulted in intact mucosa with minimal inflammatory infiltration while sloughing of gastric glands was still observed in some areas in the curative group. Significant increase in the percentage of iNOS and MDA level was found in Group III compared to control group, while significant decrease of iNOS and MDA level was noticed in Group IV and Group V as compared to Group III. Conclusion: Results of the current study revealed that administration of GSE before induction of gastric ulcer could greatly ameliorate the condition than after the induction of the ulcer.

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