Frontiers in Plant Science (May 2022)
Effects of Water Availability on Leaf Trichome Density and Plant Growth and Development of Shepherdia ×utahensis
Abstract
Many arid lands across the globe are experiencing more frequent and extreme droughts due to warmer temperatures resulting from climate change, less predictable precipitation patterns, and decreased soil moisture. Approximately 60–90% of household water is used for urban landscape irrigation in the western United States, necessitating the establishment of landscapes using drought-tolerant plants that conserve water. Shepherdia ×utahensis (hybrid buffaloberry) is a drought-tolerant plant with dense leaf trichomes (epidermal appendages) that may limit excessive water loss by transpiration. However, little is known about how S. ×utahensis regulates leaf heat balance when transpirational cooling is limited. The objective of this research was to investigate the effects of substrate water availability on plant growth and development and trichome density of S. ×utahensis. Ninety-six clonally propagated plants were grown using an automated irrigation system, and their substrate volumetric water contents were controlled at 0.05–0.40 m3·m−3 for 2 months. Results showed that water stress impaired plant growth and increased the proportion of visibly wilted leaves. Shepherdia ×utahensis acclimates to drought by reducing cell dehydration and canopy overheating, which may be accomplished through decreased stomatal conductance, smaller leaf development, leaf curling, increased leaf thickness, and greater root-to-shoot ratio. Leaf trichome density increased when stem water potential decreased, resulting in greater leaf reflectance of visible light. Cell and leaf expansion were restricted under water stress, and negative correlations were exhibited between epidermal cell size and trichome density. According to our results, plasticity in leaves and roots aids plants in tolerating abiotic stresses associated with drought. Acclimation of S. ×utahensis to water stress was associated with increased trichome density due to plasticity in cell size. Dense trichomes on leaves reflected more lights which appeared to facilitate leaf temperature regulation.
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