L-arginine and L-name effects on functional and physicochemical properties of hemoglobin in conditions of experimental chronic alcohol intoxication

Abstract

It has been revealed that different ligand forms of hemoglobin in the peripheral blood of rats are redistributed under the experimental chronic alcohol intoxication (ECAI), including a reduction of oxyhemoglobin and growth of methemoglobin against the background of total hemoglobin reduction. Also it has been found that under ECAI the concentration of alkaline resistant hemoglobin is rising, whereas the affinity of rat hemoglobin for oxygen is falling. The per os consumption of NOS substrate (L-arginine) under ECAI causes a significant decrease in methemoglobin content in the blood of rats. As for the consumption of NOS-unspecific inhibitor (L-NAME), it leads to an increase in the affinity of rat peripheral blood hemoglobin for oxygen both under ECAI and in control group. The consumption of L-arginine by rats of control group decreases the affinity of hemoglobin to oxygen similar to EСАI. The absorption spectra of RHb and HbFe2+ NO of rat’s blood were characterized at Sore band and in the ultraviolet region. It was found that rate of nitration of hemoglobin in vitro under L-arginine consumption increa­ses comparing with ECAI. In the groups of animals consumed L-NAME nitration of hemoglobin was slowed in control and in the group with ECAI.

Keywords

• experimental chronic alcohol intoxication (ECAI)
• hemoglobin
• ligand forms of hemoglobin
• hemoglobin affinity for oxygen
• NOS-unspecific inhibitor – L-NAME
• L-arginine