Gomal Journal of Medical Sciences (Aug 2017)

GENEXPERT OF CERVICAL LYMPH NODE SPECIMENS IN THE DIAGNOSIS OF EXTRAPULMONARY TB AND RIFAMPICIN RESISTANCE

  • Niaz Hussain Soomro,
  • Saifullah Baig,
  • Nisar Rao,
  • Aneeqa Ahsan Zafar,
  • Farwa Hashmat,
  • Sattar Bakhsh Awan,
  • Hashmat Ullah

Journal volume & issue
Vol. 15, no. 1

Abstract

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Background: Tuberculosis is rapidly becoming a global disease. The objective of the study was to determine the yield of the GeneXpert in the detection of DNA of mycobacterium tuberculosis and rifampicin resistance in cervical lymph node tissue specimens. Material & Methods: This cross-sectional study was conducted at the departments of Chest Medicine and Thoracic Surgery at OJHA Institute of Chest Diseases, Dow University of Health Sciences Karachi, Pakistan, from April 2014 to June 2015. The sample size was 54, selected through non-probibilty consecutive sampling technique. All patients who presented with cervical lymph adenopathy were included in the study. Demographic variables were; gender and age in years. Gender was nominal whereas age was numeric variable. Research variables were; DNA of mycobacterium TB, Rifampicin resistance. Both were nominal having attributes of positive and negative each. Data was collected and analyzed descriptively as percentages and mean along with range depending upon the data type being categorical or numeric. Descriptive statistic were calculated using SPSS Version 15. Results: Out of 54 patients, 23(42.50%) were males and 29(53.70%) females. The mean age of the patients was 32.3 years with range of 75 years (8- 83 years). Among the 54 samples tested, the GeneXpert detected the DNA of mycobacterium tuberculosis in 40 samples (74%), whereas 14(26%) were found negative. Out of 40, GeneXpert positive patients, Rifampicin resistance was detected in 2 patients (5%). And 38(95%) were Rifampicin sensitive. One of those 2 patients did not have any prior history of anti-tuberculosis therapy and was diagnosed with primary multidrug resistant tuberculosis where as the other patient being diagnosed with tuberculosis previously and completing a course of anti-tuberculosis therapy. Conclusion: GeneExpert MTB/RIF is an efficient method to detect mycobacterium TB along with Rifampicin sensitivity in cervical lymph node tissue specimens.

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