Matrix metalloprotease 9 and remodeling after myocardial infarction

Abstract

The objective of the work was to study the distribution of metalloprotease 9 (MMP9) in tissues after myocardial infarction. We studied anatomopathological material from 30 patients, the primary victims of acute transmural myocardial infarction. Average age - 63,7 years. There were 18 men and 12 women. 16 patients died in the period up to 3 days, 14 patients - in a period from 3 days to 1 month. Samples were fixed in formalin and immunohistochemical staining for MMP9 was conducted. We used rabbit IgG monoclonal antibody to MMP9 (Epitomics, Clone 1D: EP1254, Cat. N 2551-1, Lot YG 113001P) in the working dilution of 1: 100 - 1: 250. At fatal outcomes that happened within a few hours after the onset of a heart attack a large number of MMP9 was detected in neutrophils in the blood vessels at near infarction zone. In the period from 1 to 2 hours MMP9 was detected in the extracellular matrix in the area of infarction. In the period from 3 to 30 days MMP9 was detected only in fibroclasts at forming cardiosclerosis zone. Thus, the use of staining for MMP9 is convenient for determination of the time elapsed after myocardial infarction in post mortem examination.

Keywords

• myocardial infarction
• metalloprotease
• diagnostic method