Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database

Abstract

Yuanfeng Zhang,1,* Yang Chen,2,* Zhiming Chen,3 Xinye Zhou,4 Shaochuan Chen,1 Kaijian Lan,1 Zhiping Wang,5 Yonghai Zhang1 1Department of Urology, Shantou Central Hospital, Shantou, People’s Republic of China; 2Department of Nursing, Bishan Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 3Department of Pathology, Shantou Central Hospital, Shantou, People’s Republic of China; 4Centre for Reproductive Medicine, Shantou Central Hospital, Shantou, People’s Republic of China; 5Department of Urology, Lanzhou University Second Hospital, Lanzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yonghai Zhang, Email [email protected]: The extracellular matrix in the tumor microenvironment are closely related to the development of tumors. This study’s primary aim is to study the association between prolyl 3-hydroxylase 1 (P3H1) which mainly expresses collagen in extracellular matrix and the progression and prognosis of bladder cancer (BC).Methods: The clinical and transcriptome data were acquired from the cancer genome atlas database. BLCAsubtyping is used to evaluate tissue subtypes of BC. The COX proportional hazards can be used to evaluate the survival process’s influencing factors. Immunohistochemistry was used to identify differences in the expression of P3H1 in cancer and paired adjacent tissues. GSEA was used to investigate the underlying biological processes. Finally, ssGSEA, TIMER and pRRophetic were used to study the relationship between P3H1 and immune cell infiltration and drug sensitivity.Results: The expression of P3H1 was substantially higher in highly invasive BC samples than in low invasive BC. P3H1 was an independent predictor of overall survival (HR = 1.12, p = 0.03). P3H1 expression was significantly higher in tumor tissues than adjacent normal tissues in clinical tissue samples, and was significantly higher in highly stage cancer than low stage cancer samples. Samples with high P3H1 expression had a higher level of immune cell infiltration and immune function, as well as a significant correlation with macrophage and dendritic cell infiltration and TGF-beta, Th1 cells, and macrophage regulation (cor > 0.3, p < 0.05). P3H1 high expression samples were substantially more sensitive to docetaxel, cisplatin, vinblastine, camptothecin, paclitaxel, and other medicines than P3H1 low expression samples.Discussion: P3H1 is a possible oncogene and an independent predictor of poor prognosis in BC; it also has enhanced sensitivity to docetaxel, cisplatin, vinblastine, camptothecin, paclitaxel, and other medications.Keywords: prolyl 3-hydroxylase 1, bladder cancer, immune infiltration, drug sensitivity

Keywords

• prolyl 3-hydroxylase 1
• bladder cancer
• immune infiltration
• drug sensitivity.