Frontiers in Molecular Neuroscience (May 2012)

Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice

  • Mara eDierssen,
  • Laura eFedrizzi,
  • Rosa eGomez-Villafuertes,
  • Maria eMartinez de Lagran,
  • Alfonso eGutierrez-Adan,
  • Ignaci eSahun,
  • Belen ePintado,
  • Juan Carlos Oliveros,
  • Xose Manuel Dopazo,
  • Paz eGonzalez,
  • Marisa eBrini,
  • Britt eMellstrom,
  • Ernesto eCarafoli,
  • JOSE R. NARANJO,
  • JOSE R. NARANJO

DOI
https://doi.org/10.3389/fnmol.2012.00058
Journal volume & issue
Vol. 5

Abstract

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DREAM (downstream regulatory element antagonist modulator) is a Ca2+-binding protein that binds DNA and represses transcription in a Ca2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca2+ homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a severe delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.

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