A progesterone biosensor derived from microbial screening

Chloé Grazon; R C. Baer; Uroš Kuzmanović; Thuy Nguyen; Mingfu Chen; Marjon Zamani; Margaret Chern; Patricia Aquino; Xiaoman Zhang; Sébastien Lecommandoux; Andy Fan; Mario Cabodi; Catherine Klapperich; Mark W. Grinstaff; Allison M. Dennis; James E. Galagan

doi:10.1038/s41467-020-14942-5

Nature Communications (Mar 2020)

A progesterone biosensor derived from microbial screening

Chloé Grazon,
R C. Baer,
Uroš Kuzmanović,
Thuy Nguyen,
Mingfu Chen,
Marjon Zamani,
Margaret Chern,
Patricia Aquino,
Xiaoman Zhang,
Sébastien Lecommandoux,
Andy Fan,
Mario Cabodi,
Catherine Klapperich,
Mark W. Grinstaff,
Allison M. Dennis,
James E. Galagan

Affiliations

Chloé Grazon: Department of Chemistry, Boston University
R C. Baer: Department of Microbiology, Boston University
Uroš Kuzmanović: Department of Biomedical Engineering, Boston University
Thuy Nguyen: Department of Biomedical Engineering, Boston University
Mingfu Chen: Department of Biomedical Engineering, Boston University
Marjon Zamani: Department of Biomedical Engineering, Boston University
Margaret Chern: Division of Materials Science and Engineering, Boston University
Patricia Aquino: Department of Biomedical Engineering, Boston University
Xiaoman Zhang: Department of Biomedical Engineering, Boston University
Sébastien Lecommandoux: University Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629
Andy Fan: Department of Biomedical Engineering, Boston University
Mario Cabodi: Department of Biomedical Engineering, Boston University
Catherine Klapperich: Department of Biomedical Engineering, Boston University
Mark W. Grinstaff: Department of Chemistry, Boston University
Allison M. Dennis: Department of Biomedical Engineering, Boston University
James E. Galagan: Department of Microbiology, Boston University

DOI: https://doi.org/10.1038/s41467-020-14942-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

Bacteria represent an unexploited reservoir of biosensing proteins. Here the authors use genomic screens and functional assays to isolate a progesterone sensing allosteric transcription factor and use a FRET-based method to develop an optical progesterone sensor.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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