Scientific Reports (Feb 2024)

Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer

  • Mateusz Radwanski,
  • Wioletta Rozpedek-Kaminska,
  • Grzegorz Galita,
  • Natalia Siwecka,
  • Jerzy Sokolowski,
  • Ireneusz Majsterek,
  • Mutlu Özcan,
  • Monika Lukomska-Szymanska

DOI
https://doi.org/10.1038/s41598-024-54726-1
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 13

Abstract

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Abstract The aim of this study was to evaluate cytotoxicity and genotoxicity of calcium-silicate based sealers and comparing them with a gold standard—an epoxy-based sealant. Two experimental cell lines were used, gingival fibroblasts (hGF) and monocyte/macrophage peripheral blood cell line (SC). The cytotoxicity (XTT assay) and genotoxicity (comet assay) were evaluated both after 24-h and 48-h incubation. Additionally, after 48-h incubation, the cell apoptosis and cell cycle progression was detected. BioRoot Flow induced a significant decrease in hGF cells viability compared to the negative control groups both after 24-h (p 20% DNA damage). BioRoot Flow and BioRoot RCS, may have potential genotoxic effects and induce apoptosis in hGF cells which may irritate periapical tissues, resulting in a delayed healing. The findings of the study would be useful in selection of an appropriate sealant for root canal filling without causing cytotoxicity and genotoxicity.