Peculiarities of protein р53 expression in rat thymus with experimental diabetes mellitus

Abstract

Peculiarities of protein р53 expression in rat thymus with experimental diabetes mellitus were investigated and discussed in experimental work. Investigations were carried out on 28 male rats Vistar line 5-6 months of age. Experimental diabetes mellitus was designed by intraperitoneal injection of streptozotocyn (SIGMA, USA) at dose 50 mg/kg one time. Method of double immunofluorescence with usage of rat monoclonal anti-p53 and anti-СD4 antibodies was used for revelation of p53. Structure of a thymus was analyzed with the help of software VIDAS. Control animals showed an increase of p53+-cells quantity in thymus cortical zone by 58% as compared with medullar zone. Even so immature forms of p53+-cells – big lym-phocytes and p53+-lymphoblasts prevailed in population of p53+-cells of both thymus cortical zone and medullar zone. It has been established that development of experimental diabetes mellitus was accompanied by increase in number of p53+-cells and concentration of protein р53 in thymus cortical zone in the absence of any changes in medullar zone.

Keywords

• protein p53
• thymus
• apoptosis
• diabetes mellitus