Effect of sodium hypochlorite on prooxidant and antioxidant homeostasis of loach embryos during early embryogenesis

Abstract

The article contains data regarding the influence of sodium hypochlorite on the processes of free radical oxidation and antioxidant system of loach Misgurnus fossilis L. embryos in early stages of the development. It was showed that the effect of sodium hypochlorite added to the medium where the embryos develop, leads to disruption of prooxidation-antioxidant loach Misgurnus fossilis L. embryos homeostasis during early embryogenesis. It is proved that the sodium hypochlorite solution is empowered prooxydant manifestations in the early stages of embryogenesis wich are investigated and is inhibiting certain enzymes of antioxidant protection. It was found that the embryos at the 16 blastomeres stage of early embryogenesis are most sensitive to the action of sodium hypochlorite. Embryos at the 1024 blastomeres stage (10 division) are the most resistant to sodium hypochlorite as the content of lipid peroxidation products is reduced compared with control, whereas the activity of superoxidedismutase and glutathione peroxidase is within control value.

Keywords

• loach embryo
• lipid peroxidation
• superoxide dismutase
• catalase
• glutationperoxydasе
• glutathione reduced
• sodium hypochlorite