Neurobiology of Disease (Oct 2001)
A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity
- Tanya Barrett,
- Tao Xie,
- Yulan Piao,
- Ora Dillon-Carter,
- George J. Kargul,
- Meng K. Lim,
- Francis J. Chrest,
- Robert Wersto,
- Daniel L. Rowley,
- Magdalena Juhaszova,
- Li Zhou,
- Marquis P. Vawter,
- Kevin G. Becker,
- Christopher Cheadle,
- William H. Wood, III,
- Una D. McCann,
- William J. Freed,
- Minoru S. Ko,
- George A. Ricaurte,
- David M. Donovan
Affiliations
- Tanya Barrett
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Tao Xie
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Yulan Piao
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Ora Dillon-Carter
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- George J. Kargul
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Meng K. Lim
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Francis J. Chrest
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Robert Wersto
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Daniel L. Rowley
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Magdalena Juhaszova
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Li Zhou
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Marquis P. Vawter
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Kevin G. Becker
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Christopher Cheadle
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- William H. Wood, III
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Una D. McCann
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- William J. Freed
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Minoru S. Ko
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- George A. Ricaurte
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- David M. Donovan
- Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205
- Journal volume & issue
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Vol. 8,
no. 5
pp. 822 – 833
Abstract
Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter–lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.
