RESULTS OF IDENTIFICATION OF INFECTIOUS AGENTS OF INFLUENZA AND OTHER ACUTE RESPIRATORY INFECTIONS WITH THE METHOD OF RT-LAMP

Abstract

Currently, the detection and identification of viruses that cause respiratory and influenza-like illness (ILI) is one of the main tasks of public healthcare. In 2016-2017, nasopharyngeal swabs were collected from 200 sick children with ILI aged 0 to 5 years. Detection of pathogens in ILI patients was carried out by RT-LAMP amplification method. In 43 % of patients ILI viruses were detected. Among all confirmed cases of viral infection, a respiratory-syncytial virus was detected in 49 %, rhinoviruses in 16 %, coronaviruses in 9 %, parainfluenza viruses in 6 %, and human metapneumoviruses in 2 % of samples. Influenza A viruses were found in 6 % of infected specimens. The results obtained demonstrate the effectiveness of using RT-LAMP amplification in differential laboratory diagnostics of the ILI of viral etiology.

Keywords

• acute respiratory viral infections
• laboratory diagnostics
• rt-lamp