Antimicrobial activity of endophytic bacteria isolated from serrano oregano (Lippia origanoides) against Burkholderia glumae and Colletotrichum gloeosporioides

Abstract

The objective of this study was to determinate endophytic diversity associated to Lippia origanoides from Sabana subregion in the Department of Sucre, Colombia and evaluate their antimicrobial activity against Colletotrichum gloeosporioides and Burkholderia glumae. Tissue surface disinfection technique was used and isolation was performed on R2A agar culture medium. Population density (CFU / g tissue) was estimated by direct counting of colonies on plate. Statistics significances between population density in relation to tissues and site, was made by multivariate analysis and multiple range test (Tukey). Endophytic bacteria that showed inhibition of pathogenic microorganisms were subjected to an extraction of secondary metabolites. The antimicrobial, antifungal and secondary metabolites test were carried out by testing confrontation and qualitative estimation. The results show that there is a population density of endophytic bacteria associated with oregano plants and abundance is related to tissues. None of the morphotypes showed antimicrobial activity for B. glumae. One morphotypes had antifungal activity against C. gloeosporioides and metabolites extraction was performed for antifungal activity from cell suspensions.

Keywords

• Actividad antimicrobiana
• bacterias endófitas
• metabolitos secundarios.