A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds

Deepa Dehari; Aiswarya Chaudhuri; Dulla Naveen Kumar; Rohit Patil; Mayank Gangwar; Sonam Rastogi; Dinesh Kumar; Gopal Nath; Ashish Kumar Agrawal

doi:10.3390/ph16070942

Pharmaceuticals (Jun 2023)

A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds

Deepa Dehari,
Aiswarya Chaudhuri,
Dulla Naveen Kumar,
Rohit Patil,
Mayank Gangwar,
Sonam Rastogi,
Dinesh Kumar,
Gopal Nath,
Ashish Kumar Agrawal

Affiliations

Deepa Dehari: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India
Aiswarya Chaudhuri: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India
Dulla Naveen Kumar: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India
Rohit Patil: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India
Mayank Gangwar: Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India
Sonam Rastogi: Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India
Dinesh Kumar: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India
Gopal Nath: Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India
Ashish Kumar Agrawal: Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India

DOI: https://doi.org/10.3390/ph16070942
Journal volume & issue: Vol. 16, no. 7
p. 942

Abstract

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Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the A. baumannii-specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR A. baumannii clinical strains. Isolated BPABΦ1 were found to belong to the Corticoviridae family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application.

Published in Pharmaceuticals

ISSN: 1424-8247 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceuticals/

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