Automatic Quantification of Immunohistochemically Stained Cell Nuclei Based on Standard Reference Cells

Abstract

A fully automatic method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions, is presented. Agarose embedded cultured fibroblasts were fixed, paraffin embedded and sectioned at 4 µm. They were then stained together with 4 µm sections of the test specimen obtained from bladder cancer material.