Effects of Alcohol Extracts From Ganoderma resinaceum on Sleep in Mice Using Combined Transcriptome and Metabolome Analysis

Tianci Chen; Tianci Chen; Fangyi Zhang; Fangyi Zhang; Juanqin Chen; Qiangui Zhong; Qiangui Zhong; Yuxin Hu; Yuxin Hu; Ruru Wu; Baogui Xie; Yuji Jiang; Yuji Jiang; Bingzhi Chen; Bingzhi Chen

doi:10.3389/fnut.2022.745624

Frontiers in Nutrition (Jan 2022)

Effects of Alcohol Extracts From Ganoderma resinaceum on Sleep in Mice Using Combined Transcriptome and Metabolome Analysis

Tianci Chen,
Tianci Chen,
Fangyi Zhang,
Fangyi Zhang,
Juanqin Chen,
Qiangui Zhong,
Qiangui Zhong,
Yuxin Hu,
Yuxin Hu,
Ruru Wu,
Baogui Xie,
Yuji Jiang,
Yuji Jiang,
Bingzhi Chen,
Bingzhi Chen

Affiliations

Tianci Chen: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Tianci Chen: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Fangyi Zhang: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Fangyi Zhang: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Juanqin Chen: Yongtai Agricultural and Rural Bureau, Fuzhou, China
Qiangui Zhong: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Qiangui Zhong: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Yuxin Hu: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Yuxin Hu: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Ruru Wu: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Baogui Xie: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Yuji Jiang: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Yuji Jiang: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China
Bingzhi Chen: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China
Bingzhi Chen: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, China

DOI: https://doi.org/10.3389/fnut.2022.745624
Journal volume & issue: Vol. 9

Abstract

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Ganoderma resinaceum is a valuable Chinese medicine. This study aimed to investigate whether a G. resinaceum alcohol extract (GRAE) improves sleep, and analyze the potential mechanism. After 30 days of continuous administration of GRAE at various doses, GRAE (1,000 mg/kg.bw) prolonged pentobarbital sodium-induced sleep, increased the rate of sleeping in mice treated with a subthreshold dose of pentobarbital sodium, and shortened sleep latency. The mice brain was analyzed using UPLC-MS/MS and RNA-sequencing. Metabolomics analysis revealed that 73 metabolites in the high-dose (HD) group had changed significantly, mainly in amino acids and their derivatives, especially the accumulation of L-glutamine and PGJ2 (11-oxo-15S-hydroxy-prosta-5Z, 9, 13E-trien-1-oic acid). Transcriptome analysis revealed 500 differential genes between HD and control groups, mainly enriched in neuroactive ligand-receptor interaction, amphetamine addiction, and cocaine addiction pathways. The conjoint analysis of the transcriptome and metabolome showed that the biosynthesis of L-glutamine might be regulated by Homer1, Homer3, and Grin3b. This suggests that GRAE may affect L-glutamine accumulation by regulating the expression of these genes. This study showed that GRAE may prolong the sleep time of mice by reducing the accumulation of L-glutamine and deepens our understanding of the regulatory network between certain genes and L-glutamine.

Published in Frontiers in Nutrition

ISSN: 2296-861X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.frontiersin.org/journals/nutrition/

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