Estimation of Phytochemical Constituents and Evaluation of Antioxidant Potency of Piper betle Leaves

Abstract

Betel leaves (Piper betle) are a species of Piper genus which is rich in phytonutrients like steroids, phenols, flavonoids, alkaloids, steroids, saponins and terpenoids. Anti-cancer, antibacterial, anti-inflammatory, antioxidant, anti-mutagenic, antiplaque, and anti-diabetic properties are among the health benefits of this plant's leaf extract. This study aimed to quantify the phytochemical constituents and assess the antioxidant capacity of piper betle leaves. In this study, qualitative phytochemical screening were used done by using test for alkaloids, glycosides, phenols, flavonoids, steroids, terpenoids, saponins. Additionally, we calculated the betel leaves' total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity using DPPH, H202 scavenging activity after extraction by methanol using Soxhlet apparatus. The TPC and TFC was noted 51.5278 mg GAE/gm and 33.769 mg QE/gm respectively. There was a significant positive connection found between DPPH and Total Phenolic Content. (R²=0.9939) (p value= 0.0002), H202 (R²=0.9979) (p value<0.0001) respectively at a 95% confidence level which is consistent with the above literature reports. There is significant relationship between TFC and DPPH (R²=0.9781) (p value= 0.0014) H202 (R²=0.9591) (p value= 0.0036) respectively at a 95% confidence level. The leaves of Piper betle contain high amount of phenolic and flavonoid content, hence can be used as natural antioxidant. To fully comprehend antioxidants' potential to prevent diseases that have a major negative influence on life expectancy, more research must be done on the separation, identification, and mechanism of action of the relevant antioxidant components.

Keywords

• Phenolic
• Flavonoid
• , Antioxidant
• Correlation
• Piper betle