BioTechnologia (Mar 2022)
Antioxidant and antimicrobial potentials of mycelial extracts of Hohenbuehelia myxotricha grown in different liquid culture media
Abstract
In addition to their nutritional properties, mushrooms have emerged as a health supplement because of their me di cinal potential. Many studies have shown that mushrooms exhibit important biological activities. Here, the anti oxi dant and antimicrobial activities of Hohenbuehelia myxotricha (Lév.) Singer mycelia cultivated on Sabouraud dext rose broth (SDB) and glucose peptone yeast (GPY) medium were studied. The total antioxidant status (TAS), total oxi dant status (TOS), and oxidative stress index (OSI) of ethanolic extracts of mycelia were measured using Rel Assay kits. The antioxidant and oxidant potentials of H. myxotricha mycelial extracts were determined for the first time in the present study. The highest TAS, TOS, and OSI values of H. myxotricha were 5.416±0.150 mmol/l, 1.320±0.156 μmol/l, and 0.024±0.003, respectively. Ethanolic mycelial extracts of H. myxotricha showed anti microbial activities at concentrations from 25 to 200 μg/ml against all the studied bacteria (Acinetobacter bau mannii, Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, methicillin-resistant S. aureus, and Pseudo monas aeruginosa) and fungi (Candida albicans, C. glabrata, and Issatchenkia orientalis) tested by the agar dilution method. The antifungal activity of the extract was more significant than its antibacterial activity. The anti oxidant, oxidant, and antimicrobial potentials of H. myxotricha mycelia varied depending on the culture media used. GPY medium was more suitable for the synthesis of antibiotic compounds against E. coli, while SDB me dium was more appropriate for producing metabolites with antioxidant and antifungal properties. Based on the results, ethanolic extract of H. myxotricha mycelia showed a significant pharmacological potential and could be used as a natural antioxidative and antimicrobial source for health benefit.
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