Проблемы особо опасных инфекций (Apr 2012)

Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen

  • S. V. Generalov,
  • E. G. Abramova,
  • Zh. V. Matveeva,
  • I. M. Zhulidov,
  • O. A. Lobovikova,
  • L. V. Savitskaya,
  • L. N. Minaeva,
  • M. V. Galkina,
  • T. A. Mikheeva,
  • A. V. Komissarov,
  • M. N. Kireev,
  • A. K. Nikiforov

DOI
https://doi.org/10.21055/0370-1069-2012-2(112)-78-81
Journal volume & issue
Vol. 0, no. 2(112)
pp. 78 – 81

Abstract

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Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin.

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