A wide survey of heavy metals-induced in-vitro DNA replication stress characterized by rate-limited replication

Qidong Ren; Xuejun Guo; Dong Yang; Chuanfang Zhao; Xiangyuan Zhang; Xinghui Xia

Current Research in Toxicology (Jan 2024)

A wide survey of heavy metals-induced in-vitro DNA replication stress characterized by rate-limited replication

Qidong Ren,
Xuejun Guo,
Dong Yang,
Chuanfang Zhao,
Xiangyuan Zhang,
Xinghui Xia

Affiliations

Qidong Ren: Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, 23788 Gongye North Road, Jinan 250100, China; State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China
Xuejun Guo: State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China; Corresponding authors.
Dong Yang: Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing 100875, China; Corresponding authors.
Chuanfang Zhao: State Key Laboratory of Environmental Chemistry and Eco-Toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Xiangyuan Zhang: State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China
Xinghui Xia: State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China

Journal volume & issue: Vol. 6
p. 100152

Abstract

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Heavy metals (HMs) are environmental pollutants that pose a threat to human health and have been accepted to cause various diseases, including cancer and developmental disorders. DNA replication stress has been identified to be associated with such diseases. However, the effect of HMs exclusively on DNA replication stress is still not well understood. In this study, DNA replication stress induced by thirteen HMs was assessed using a simplified in-vitro DNA replication model. Two parameters, Cte/Ctc reflecting the cycle threshold value alteration and Ke/Kc reflecting the linear phase slope change, were calculated based on the DNA replication amplification curve to evaluate the rate of exponential and linear phases. These parameters were used to detect the replication rate reflecting in-vitro DNA replication stress induced by tested HMs. According to the effective concentrations and rate-limiting degree, HMs were ranked as follows: Hg, Ce > Pb > Zn > Cr > Cd > Co > Fe > Mn, Cu, Bi, Sr, Ni. Additionally, EDTA could relieve the DNA replication stress induced by some HMs. In conclusion, this study highlights the potential danger of HMs themselves on DNA replication and provides new insight into the possible links between HMs and DNA replication-related diseases.

Published in Current Research in Toxicology

ISSN: 2666-027X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Public aspects of medicine: Toxicology. Poisons
Website: https://www.journals.elsevier.com/current-research-in-toxicology

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