Journal of King Saud University: Science (Oct 2021)
To investigate the expression of miRNA in nicotine induced GISTs tumor rats and the mitigation of cardiovascular complications
Abstract
The common for the most part of tumor (GISTs) Gastrointestinal stromal tumor is known mesenchymal neoplasms of the tumor in gastrointestinal tract. Detection of serum miRNA 1, 15b, 21 adjustment mainly cardio marker enzymes and their mechanism of action and heart tissue, observed changes in LDH, CK-MB, AST, ALT, lipid peroxidation (LPO) and Troponin T estimations/biochemical changes observed in serum of experimental subjects (nicotine treated rats, 0.6 mg/kg, i.p.). In the present study, gastrointestinal stromal tumors of rats and the mitigation of cardiovascular complications was experimentally induced in rats by nicotine i.p. treatment of adult rats and maintained control rats. Confirm significantly decreased expression of miRNA-1 (nicotine induced gastrointestinal tumor rats), the levels of miRNA-15b expression is decrease in group II but miRNA-21 expression values are increased in experimental rats (group II) with control rats (group I). Body weight decreased significantly in group II nicotine induced rats and heart weight increased in group II but systolic blood pressure is decreased in experimental rats (group II) with control rats. Cardio marker enzyme levels were measured and increases LDH levels, slight raises CK-MB concentration range in group II. AST, ALT marker enzyme levels are higher in experimental rats with controls, troponin T values are clearly declare that some cardio complications in group II. Observed Ldh, Ck-Mb, Ast and Alt levels are decreased followed by antioxidant enzymes SOD, catalase but lipidperoxidation and protein carbonyls values are increased in nicotine induced rats and match up to normal rats. GST levels are increases significantly and Gpx, GRd and GSH levels are decreased in group II nicotine induced rats and corporately control group I. This study to investigates, for the first time, the effect of nicotine i.p., in adult rats (nicotine treated) and control rats. Reports of serum as well as heart tissue adjustment in molecular levels.
