Molecular and genetic studies of the basis of virulence/avirulence in Meloidogyne chitwoodi

Abstract

Within the EU project DREAM there is a programme of work investigating the molecular basis of virulence and avirulence in Meloidogyne chitwoodi and which involves partners from the Netherlands, France and Scotland. In Wageningen AFLPs are being used to examine genetic diversity between populations of M. chitwoodi. Partners in Wageningen and INRA are using M. javanica and M. incognita as model systems to isolate genes producing secreted proteins that could be implicated in the host pathogen interaction. They will search for homologous sequences in M. chitwoodi. At Rennes two-dimensional electrophoresis (2DE) studies are being conducted. The PCR based suppressive subtractive hybridisation (SSH), has been used at PRI and SCRI to compare avirulent and virulent nematodes and infected plant material (resistant and susceptible) at different times after infection.

Keywords

• meloidogyne chitwoodi
• potato
• aflp
• cdna
• suppressive subtractive hybridisation (ssh)
• two-dimensional electrophoresis (2de)