Química Nova (Jan 2012)

A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

  • Renata F. J. Tiossi,
  • Juliana C. Da Costa,
  • Mariza A. Miranda,
  • Fabíola S. G. Praça,
  • Maria Vitória L. B. Bentley,
  • Jairo K. Bastos,
  • James D. McChesney

DOI
https://doi.org/10.1590/S0100-40422012001100038
Journal volume & issue
Vol. 35, no. 11
pp. 2312 – 2316

Abstract

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To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.

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