Surface Functionalization of Poly(l-lactide-co-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering

Anna M. Tryba; Małgorzata Krok-Borkowicz; Michał Kula; Natalia Piergies; Mateusz Marzec; Erik Wegener; Justyna Frączyk; Rainer Jordan; Beata Kolesińska; Dieter Scharnweber; Czesława Paluszkiewicz; Elżbieta Pamuła

doi:10.3390/jfb13010004

Journal of Functional Biomaterials (Jan 2022)

Surface Functionalization of Poly(l-lactide-co-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering

Anna M. Tryba,
Małgorzata Krok-Borkowicz,
Michał Kula,
Natalia Piergies,
Mateusz Marzec,
Erik Wegener,
Justyna Frączyk,
Rainer Jordan,
Beata Kolesińska,
Dieter Scharnweber,
Czesława Paluszkiewicz,
Elżbieta Pamuła

Affiliations

Anna M. Tryba: Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland
Małgorzata Krok-Borkowicz: Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland
Michał Kula: Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland
Natalia Piergies: Institute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Kraków, Poland
Mateusz Marzec: Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland
Erik Wegener: Faculty of Chemistry and Food Chemistry, Technische Universität Dresden, Mommsenstr, 401069 Dresden, Germany
Justyna Frączyk: Faculty of Chemistry, Institute of Organic Chemistry, Łódź University of Technology, ul. Żeromskiego 116, 90-924 Łódź, Poland
Rainer Jordan: Faculty of Chemistry and Food Chemistry, Technische Universität Dresden, Mommsenstr, 401069 Dresden, Germany
Beata Kolesińska: Faculty of Chemistry, Institute of Organic Chemistry, Łódź University of Technology, ul. Żeromskiego 116, 90-924 Łódź, Poland
Dieter Scharnweber: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, Germany
Czesława Paluszkiewicz: Institute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Kraków, Poland
Elżbieta Pamuła: Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland

DOI: https://doi.org/10.3390/jfb13010004
Journal volume & issue: Vol. 13, no. 1
p. 4

Abstract

Read online

Bone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(l-lactide-co-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For a novel method of membrane manufacture, the RGD motifs were coupled with the non-ionic amphiphilic polymer poly(2-oxazoline) (POx). The RGD-containing membranes were then prepared by solvent casting of PLGA, POx coupled with RGD (POx_RGD), and poly(ethylene glycol) (PEG) solution in methylene chloride (DCM), followed by DCM evaporation and PEG leaching. Successful coupling of RGD to POx was confirmed spectroscopically by Raman, Fourier transform infrared in attenuated reflection mode (FTIR-ATR), and X-ray photoelectron (XPS) spectroscopy, while successful immobilization of POx_RGD on the membrane surface was confirmed by XPS and FTIR-ATR. The resulting membranes had an asymmetric microstructure, as shown by scanning electron microscopy (SEM), where the glass-cured surface was more porous and had a higher surface area then the air-cured surface. The higher porosity should support bone tissue regeneration, while the air-cured side is more suited to preventing soft tissue infiltration. The behavior of osteoblast-like cells on PLGA membranes modified with POx_RGD was compared to cell behavior on PLGA foil, non-modified PLGA membranes, or PLGA membranes modified only with POx. For this, MG-63 cells were cultured for 4, 24, and 96 h on the membranes and analyzed by metabolic activity tests, live/dead staining, and fluorescent staining of actin fibers. The results showed bone cell adhesion, proliferation, and viability to be the highest on membranes modified with POx_RGD, making them possible candidates for GTR applications in periodontology and in bone tissue engineering.

Published in Journal of Functional Biomaterials

ISSN: 2079-4983 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology; Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/jfb/

About the journal

Abstract

Keywords