Validation of the method for determining lincomycin levels and calculating lincomycin levels in broiler chicken plasma using High-Performance Liquid Chromatography

Abstract

Background: Antibiotic residues come from food of animal origin, such as broiler chicken, have a variety of consequences on human health and increase the likelihood of antibiotic resistance. Lincomycin residue investigations in broiler chicken especially in plasma broiler chicken should be undertaken utilising the validation method analysis. Aim: The purpose of this study is to determine the high-performance liquid chromatography (HPLC) as a validation method for calculating the residual concentration of lincomycin in broiler chicken blood plasma and compare it with the Minimum Inhibitor Concentration (MIC) and Maximum Residue Limits (MRL) standards for lincomycin. Methods: Thirty-five-day-old broiler chickens cobb 700 were weighed and randomly allocated to and separated into control (placebo) and six treatment groups of varying doses and duration. The treatment group's suggested dosage of lincomycin was 50, 100, or 150 mg/kg/day given to 18-day-old chicken, along with drinking water for a week (A group) and two weeks (P group). Lincomycin levels in blood plasma were validated using HPLC. The residual lincomycin concentrations 24 hours and one week after injection were compared to the lincomycin MIC and the Indonesian National Standard of MRL. Result: The validation of lincomycin reveals a linear value in blood plasma with an R2 of 0.9983. Precision and accuracy levels indicate promising results for detecting lincomycin. The retention duration for 100 µg/mL lincomycin was 10.0-10.5 minutes. Lincomycin had LOD and LOQ values of 13.98 and 4.86 µg/mL, respectively. After one week of dosing at 50 and 100 mg/kg dosages, lincomycin residue detection was 0.00, which was below the MRL criterion of [Open Vet J 2024; 14(6.000): 1453-1459]

Keywords

• broiler chicken
• high-performance liquid chromatography
• lincomycin
• plasma
• residue