Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range

Abstract

SpCas9 is a versatile genome-editing tool, but it is large and cannot be packaged efficiently in a AAV vector, limiting its application. The reported engineered Campylobacter jejuni Cas9 variant exhibits enhanced cleavage activity and a broader targeting range, expanding the CRISPR-Cas toolbox for therapeutic genome engineering.